Nic gonadotropin (hCG; Sankyo Zoki, Tokyo, Japan), and 10 (v/v) porcine
Nic gonadotropin (hCG; Sankyo Zoki, Tokyo, Japan), and ten (v/v) porcine follicular fluid (pFF). The pFF was collected from antral follicles of prepubescent gilts, centrifuged at 1,600g for 30 min…