A, P 0.05 vs. control NPD-fed mice. B: Ceramides, 2-way ANOVA for ceramides: genotype P = 0.37; diet P = 0.18; P genotype ?diet program = 0.18,and C: DAG had been measured using LC/MS/MS technique as described inside the techniques section, n= 5? per group. 2-way ANOVA for DAG: genotype P = 0.42; diet plan P = 0.54; P genotype ?eating plan = 0.83.NIH-PA Author ManuscriptJ Cardiovasc Pharmacol. Author manuscript; available in PMC 2014 April 01.Khan et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 6. Effects of FO on MHC-PPAR miceA. Fractional shortening determined by echocardiography was made use of to assess cardiac function in 14-wk old control and MHC-PPAR mice fed non-purified diet (NPD) or low dose (LD) FO for 6 weeks. n = 8? mice per group. 2-way ANOVA for FS: genotype P=0.12; diet regime P = 0.07; genotype ?diet plan P = 0.ten. a, P 0.05 vs. control NPD-fed mice. B: PKC activation in handle and MHC-PPAR transgenic mice, measured working with immunoprecipitation with PKC antibodies as described within the methods section, n= four? per group. C: Survival curve of MHC-PPAR mice, n=33?two per group.J Cardiovasc Pharmacol. Author manuscript; accessible in PMC 2014 April 01.TABLEEffect of fish oil on physique weight and plasma metabolitesNPD Controls 21.3 ?0.four 4.1 ?0.1 7.four ?0.3 1.four ?0.1 0.9 ?0.1 two.9 ?0.1 2.7 ?0.1 three.0 ?0.1 3.1 ?0.1 2.four ?0.1 two.five ?0.1 0.0001 1.0 0.29 0.eight ?0.1 0.7 ?0.2 0.six ?0.1 0.eight ?0.0 0.9 ?0.1 0.55 0.10 0.70 1.six ?0.1 1.three ?0.1 1.three ?0.1 1.1 ?0.1 1.2 ?0.1 0.0099 0.27 0.66 7.five ?0.2 8.1 ?0.two eight.three ?0.two 7.six ?0.three six.eight ?0.six 0.0149 0.55 0.28 4.1 ?0.1 3.7 ?0.1 three.eight ?0.1 two.7 ?0.2 two.5 ?0.two 0.0001 0.72 0.56 21.7 ?0.three 20.7 ?0.4 22.0 ?0.6 22.1 ?0.three 21.six ?0.two 0.82 0.17 0.33 ACS1 Controls ACS1 Controls ACS1 Eating plan Genotype Interaction LD FO HD FO P (ANOVA)Body weight, gFood intake, gGlucose, mmol/LTG, mmol/LFree FA, mMTC, mmol/LJ Cardiovasc Pharmacol.(S)-3-Fluoropyrrolidine (hydrochloride) web Author manuscript; offered in PMC 2014 April 01.2,4-Dichloro-8-fluoroquinazoline structure Values are means ?SEM.PMID:35116795 Manage and MHC-ACS1 (ACS1) mice had been fed non-purified diet (NPD), LD or HD FO diet for six weeks. Measurements of plasma glucose, TG, free of charge FA, TC have been taken immediately after an overnight speedy. Statistics have been performed making use of 2-way ANOVA. N = five?5 mice per group.NIH-PA Author ManuscriptKhan et al. PageNIH-PA Author ManuscriptNIH-PA Author ManuscriptTABLEFatty acid (FA) composition of selected diacylglycerolsHD FO ACS1 312 ?17 two.six ?0.two 13.2 ?0.7 a 9.7 ?0.7 11.2 ?1.1 23.7 ?6.2 16.2 ?two.7 six.7 ?1.six 855 ?129 947 ?116 0.32 0.54 0.89 2.0 ?0.two 0.06 0.0005 0.13 ten.four ?1.1 0.0026 0.0199 0.19 14.six ?6.6 0.09 0.58 0.18 six.7 ?0.eight 0.0022 0.0064 0.97 0.0006 0.0061 0.0010 11.9 ?0.9 12.9 ?1.0 a* 7.two ?0.five 1.7 ?0.1 a* 1055 ?118 9.three ?2.0 b 1.five ?0.1 1.9 ?0.2 0.0084 0.0084 0.43 213 ?three 294 ?34 b* 0.73 0.0008 0.67 Controls ACS1 Diet Genotype Interaction P (ANOVA)Khan et al.NPDFA species, nmol/gControlsC16/C18:211 ?C18/C20:1.9 ?0.C18/C9.8 ?0.C18:2/C16.3 ?2.C20:4/C20:9.0 ?1.C20:5/C20:9.0 ?1.C20:5/C22:3.eight ?0.Total997 ?Data are mean ?SEM. Control and MHC-ACS1 (ACS1) mice have been fed either NPD or HD FO diet for 6 weeks. Right after an overnight fast, DAG was extracted from 100mg of frozen hearts as described in strategies. FA composition of DAG have been identified by LCMS/MS. Statistics were performed utilizing 2-way ANOVA. N=5? mice per group.aP 0.05 vs. Control NPD fed mice.a*P 0.05 vs. Handle NPD fed mice (by t-test).bP 0.01 vs. Manage HD FO fed mice.J Cardiovasc Pharmacol. Author manuscript; accessible in PMC 2014 April 01.b*P 0.05 vs. ACS1 NPD fed mice by t-test).NIH-PA Author ManuscriptPageNIH-PA Author Manuscript.
