Al society; AUC: Area beneath the curve; CHO: Chinese hamster ovarian; Cmax: Maximum concentration; CQ: Chloroquine; CV: Coefficient of variation; EMA: European Medicines Agency; FDA: Food and Drug Administration; HPLC: Higher functionality liquid chromatography; IC50: 50 inhibitory concentration; IS-MF: Internal standard normalized matrix aspect; IV: Intravenous; LC-MS/MS: Liquid chromatography tandem massConclusion Robust LC-MS/MS procedures were created and validated for the quantification of TK900D and TK900E in blood, applying a very small extraction volume (20 l).Abay et al. Malaria Journal 2014, 13:42 http://malariajournal/content/13/1/Page 13 ofspectrometer; LLE: Liquid-liquid extraction; LLOQ: Decrease limit of quantification; MMV: Medicines for Malaria Venture; MRM: A number of reaction monitoring; MTT: (3-(four, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide; Nom: Nominal; OIS: On-instrument stability; PK: Pharmacokinetic; QC: High-quality control; S/N: Signal-to-Noise ratio; SPVS: System performance verification sample; ULOQ: Upper limit of quantification. Competing interests The authors declare that they have no competing interests. Authors’ contributions ETA Developed and validated the LC-MS/MS assay for the quantitative determination of TK900D and TK900E in mouse blood, and made use of the assay for PK-evaluation with the analytes; performed the data acquisition and interpretation on the outcomes presented in the manuscript; compiled data and presented it in the form as it appears inside the manuscript. MT synthesized the compounds and supplied us with in vitro activity data. LG assisted with the evaluation from the PK-properties using PK-summit computer software.Imidazo[1,2-a]pyridine-8-carbaldehyde web LW, KJS and JHW edited, revised and accepted the manuscript, which is part of ETA’s PhD project. KC revised the manuscript. The final version of the manuscript has been read and accepted by each of the authors. Acknowledgments We would like to acknowledge the following institutions for their contribution for the completion of this study: PAREXEL International clinical investigation organization, Bloemfontein, South Africa, where the analytical function was performed; the PK laboratory as well as the animal unit on the pharmacology division at the University of Cape Town, exactly where the animal work was accomplished; the University on the Cost-free State as well as the Technologies and Human Resources for Industry Programme (THRIP) for financial help; the University of Cape Town, the South African Medical Study Council plus the South African Research Chairs initiative from the Department of Science and Technologies, administered by means of the South African National Analysis Foundation are gratefully acknowledged for assistance (KC); the South African Medical Study Council for economic help (self-initiated research grant ?Lubbe Wiesner).Methyl 5-formylpicolinate web Author specifics 1 Division of Clinical Pharmacology, Division of Medicine, University of Cape Town, Observatory, 7925 Cape Town, South Africa.PMID:23771862 2PAREXEL?International Clinical Analysis Organisation, Private Bag X09, Brandhof 9300, Bloemfontein, South Africa. 3Department of Chemistry, University in the Free of charge State, PO Box 339, Bloemfontein 9300, South Africa. 4Department of Chemistry, University of Cape Town, Rondebosch 7701, Cape Town, South Africa. 5Institute of Infectious Illnesses and Molecular Medicine, University of Cape Town, Rondebosch 7701, Cape Town, South Africa. Received: 19 November 2013 Accepted: 28 January 2014 Published: 31 January 2014 References 1. Planet Well being Organization Media Centre: Malaria Fact.